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目的探讨贝那普利和氨氯地平对自发性高血压大鼠(SHR)胆囊收缩素(CCK)mRNA及蛋白表达的影响。方法 45只14周龄雄性SHR随机分为SHR组(n=15)、贝那普利组[盐酸贝那普利灌服0.90mg/(kg·d),n=15]、氨氯地平组[苯磺酸氨氯地平灌服0.45mg/(kg·d),n=15],另以Wistar-Kyoto(WKY)大鼠作为正常对照组(n=15),SHR组及WKY组每日灌服同体积的蒸馏水,干预8周,测定各组大鼠安静清醒状态下尾动脉压,应用酶联免疫吸附试验(ELISA)法检测血清中CCK的含量;采用逆转录聚合酶链反应(RT-PCR)检测十二指肠中CCK mRNA表达;采用Western blot法检测十二指肠和脑中CCK蛋白表达。结果干预8周后,与WKY组比较,SHR组血压升高[收缩压/舒张压(201.0±14.6)/(157.4±16.2)比(136.3±12.0)/(102.9±10.2)mm Hg],十二指肠中CCK mRNA和蛋白表达显著降低,脑中CCK蛋白表达显著降低(均P<0.05),但血清CCK水平差异无统计学意义(P>0.05);与SHR组比较,贝那普利组与氨氯地平组血压降低[(181.5±17.5)/(145.4±18.3)、(188.4±14.5)/(145.6±13.8)比(201.0±14.6)/(157.4±16.2)mm Hg,均P<0.05],贝那普利组与氨氯地平组十二指肠中CCK mRNA和蛋白表达、脑中CCK蛋白表达显著升高(均P<0.05)。结论 SHR十二指肠和脑组织中CCK调节失衡,贝那普利和氨氯地平可能通过调节CCK的表达而降压。
Objective To investigate the effects of benazepril and amlodipine on the expression of cholecystokinin (CCK) mRNA and protein in spontaneously hypertensive rats (SHR). Methods Forty-five male SHRs were randomly divided into SHR group (n = 15), benazepril group (benazepril hydrochloride 0.90 mg / (kg · d), n = 15] (Amlodipine besylate orally 0.45mg / (kg · d), n = 15], and Wistar-Kyoto (WKY) rats as normal control group (n = 15) The same volume of distilled water was fed in for 8 weeks. The tail arterial pressure of rats in each group was measured in a quiet and awake state. The content of CCK in serum was detected by enzyme linked immunosorbent assay (ELISA) -PCR) was used to detect the expression of CCK mRNA in duodenum. The expression of CCK protein in duodenum and brain was detected by Western blot. Results Compared with the WKY group, the blood pressure (systolic / diastolic pressure (201.0 ± 14.6) / (157.4 ± 16.2) vs (136.3 ± 12.0) / (102.9 ± 10.2) mmHg] The expression of CCK mRNA and protein in the duodenum was significantly decreased, and the expression of CCK protein in the brain was significantly decreased (all P <0.05), but there was no significant difference in serum CCK levels (P> 0.05). Compared with SHR group, (P <0.01), and the blood pressure in the amlodipine group was significantly lower than that in the amlodipine group [(181.5 ± 17.5) / (145.4 ± 18.3), (188.4 ± 14.5) / (145.6 ± 13.8) vs (201.0 ± 14.6) / (157.4 ± 16.2) 0.05]. The expressions of CCK mRNA and protein in duodenum of benazepril group and amlodipine group were significantly increased (all P <0.05). Conclusions The CCK regulation in duodenum and intestine of SHR is unbalanced, and benazepril and amlodipine may be depressurized by regulating the expression of CCK.