论文部分内容阅读
利用同-PCR反应体系,对分别与番茄抗根结线虫的Mi基因和抗斑萎病毒(TSWV)的Sw-5基因紧密连锁的SCAR标记进行了同时扩增筛选,扩增的特异性片段与单引物扩增片段完全吻合,其中与Mi基因紧密连锁的SCAR1标记为共显性标记,抗感试材均产生750 bp的特异片段,纯合和杂合抗病基因型试材存在rqp I酶切位点,酶切后分别产生了570 bp、160 bp和750 bp、570 bp、160 bp的不同特异性片段,而感病基因型试材无Taq I酶切位点;与Sw-5基因紧密连锁的SCAR2标记为显性标记,只有抗病试材扩增出400 bp的特异性片段。经反复验证,结果稳定准确可靠,可用于在同-PCR反应体系中对两个抗病基因进行同时筛选鉴定。
The SCAR markers closely linked to the Sw genes of Mi root-knot nematode and Verticillium wilt (TSWV) were respectively amplified and screened by the same-PCR reaction system. The amplified fragments The single-primer amplified fragment was completely consistent. The SCAR1 marker closely linked to the Mi gene was labeled as a co-dominant marker, and the specific resistance fragments were all 750 bp. The homozygous and heterozygous resistant genotypes had rqp I enzyme The specific fragments of 570 bp, 160 bp and 750 bp, 570 bp and 160 bp were generated after cleavage and cleavage, respectively. But Taq I restriction sites were not found in the susceptible genotypes, The tightly linked SCAR2 marker is a dominant marker, and only the 400 bp specific fragment was amplified from disease-resistant specimens. After repeated verification, the results are stable, accurate and reliable and can be used for simultaneous screening and identification of two disease-resistance genes in the same-PCR reaction system.