[Objective] To study the microwave-assisted cyclohexane optimum extraction conditions of essential oils from the stem of Paederia scandens and analyze their content by GC-MS, so as to provide references for the scientific utilization of P. scandens. [Method] The essential oils were first extracted from the stem of P. scandens by microwave-assisted cyclohexane extraction and then analyzed by GC-MS. The optimum conditions were screened by orthogonal experiment. [Result] The optimum extraction conditions were as follows: MP. scandens stem∶ Vcyclohexane=2∶ 60 (g/ml), irradiation time 10 min, microwave power 800 W and irradiation temperature 50 ℃, under which the yield of essential oils reached 1.32%. 10 compositions were isolated from the stem extracts of P. scandens by GC-MS, of which seven compositions accounting for 90.690% of the total peak area were identified, mainly including 18.424% n-palmitic acid, 24.243% phthalic acid isobutyl nonyl ester and 15.131% oleic acid. [Conclusion] The microwave-assisted cyclohexane extraction method was rapid and efficient to extract essential oils from the stem of P.scandens, but that remain needed to be further improved because of the too few isolated components. [Objective] To study the microwave-assisted extraction of essential oils from the stem of Paederia scandens and analyze their content by GC-MS, so as to provide references for the scientific utilization of P. scandens. [Method] The essential oils were first extracted from the stem of P. scandens by microwave-assisted cyclohexane extraction and then analyzed by GC-MS. The optimum conditions were screened by orthogonal experiment. [Result] The optimum extraction conditions were as follows: MP. scandens stem: Vcyclohexane = 2:60 (g / ml), irradiation time 10 min, microwave power 800 W and irradiation temperature 50 ° C, under which the yield of essential oils reached 1.32%. 10 compositions were isolated from the stem extracts of P. scandens by GC-MS, of which seven compositions accounting for 90.690% of the total peak area were identified, mainly including 18.424% n-palmitic acid, 24.243% phthalic acid isobutyl nonyl ester and 15.131% oleic acid. [Conclusio n] The microwave-assisted cyclohexane extraction method was rapid and efficient to extract essential oils from the stem of P.scandens, but that remained needed to be further improved because of the too few isolated components.