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目的探讨食管鳞状细胞癌中细胞黏附分子l(CADM1)mRNA的表达及其临床病理学意义。方法采用RT-PCR半定量检测50例食管鳞癌(食管鳞癌组)及其邻近正常食管黏膜(正常黏膜组)CADM1 mRNA的表达。结果食管鳞癌组CADM1 mRNA表达量低于正常黏膜组(0.522±0.247vs.0.871±0.192)(P<0.01)。食管鳞癌组有淋巴结转移患者的CADM1mRNA表达量低于无淋巴结转移者(P<0.01)。CADM1 mRNA表达与食管鳞癌的TNM分期密切相关(P<0.05),而与患者性别、年龄及肿瘤组织学分级无相关性(P>0.05)。结论 CADM1mRNA在正常食管黏膜组织中高表达,而在食管鳞状细胞癌中低表达,提示CADM1表达缺失对在食管鳞状细胞癌的发生、侵袭、转移有一定的作用;检测CADM1mRNA表达对判断食管鳞状细胞癌患者预后有一定价值。
Objective To investigate the expression of cell adhesion molecule-1 (CADM1) mRNA in esophageal squamous cell carcinoma and its clinicopathological significance. Methods Semi-quantitative RT-PCR was used to detect the expression of CADM1 mRNA in 50 esophageal squamous cell carcinomas (ESCC) and adjacent normal esophageal mucosa (normal mucosa). Results The expression of CADM1 mRNA in esophageal squamous cell carcinoma was lower than that in normal mucosa (0.522 ± 0.247 vs 0.871 ± 0.192) (P <0.01). The expression of CADM1mRNA in patients with esophageal squamous cell carcinoma with lymph node metastasis was lower than that without lymph node metastasis (P <0.01). The expression of CADM1 mRNA was closely related to the TNM stage of esophageal squamous cell carcinoma (P <0.05), but not to the gender, age and histological grade (P> 0.05). Conclusions The high expression of CADM1 mRNA in normal esophageal mucosa tissues and low expression in esophageal squamous cell carcinoma suggest that the loss of CADM1 gene may play an important role in the occurrence, invasion and metastasis of esophageal squamous cell carcinoma. To detect the expression of CADM1 mRNA in esophageal squamous cell carcinoma The prognosis of squamous cell carcinoma has some value.