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本文采用杂交瘤技术，制备了７ 株抗甲型副伤寒沙门氏菌鞭毛抗原单克隆抗体（ｍ Ａｂ） 的杂交瘤细胞株。对３ 株ｍＡｂ（２Ｃ８、２Ｅ６ 和５Ｄ７） 进行了鉴定，ｍＡｂ ２Ｃ８ 和５Ｄ７ 为ＩｇＭ，２Ｅ６ 为ＩｇＧ１（κ）亚类，可分别识别不同的抗原表位，均具有很强的特异性，即只与甲型副伤寒沙门氏菌及其鞭毛抗原起反应，而与相关肠道细菌：尼亚里木沙门氏菌、达卡沙门氏菌及伤寒沙门氏菌、乙型和丙型副伤寒杆菌、猪霍乱沙门氏菌、纽波特沙门氏菌等均无交叉反应。免疫印迹显示，３ 株ｍＡｂ 只能与甲型副伤寒沙门氏菌鞭毛抗原Ｍｒ 为５２ ０００ 的蛋白结合。用ｍＡｂ ２Ｅ６ 和５Ｄ７ 建立的双ｍＡｂ 夹心ＥＬＩＳＡ 法，检测了１３ 例血培养阳性的副伤寒患者血清中的甲型副伤寒沙门氏菌鞭毛抗原，１２ 例呈阳性，阳性符合率为９２－３％ 。
In this paper, hybridoma technology was used to prepare seven hybridoma cell lines with monoclonal antibodies against Salmonella Paratyphi A (m Ab). Three mAbs (2C8, 2E6 and 5D7) were identified, mAb 2C8 and 5D7 were IgM, and 2E6 was IgG1 (κ) subclass, which could recognize different epitopes respectively and all showed strong specificity Reacted with Salmonella paratyphi A and its flagellar antigens, but not with the associated enteric bacteria, Salmonella nidulans, Salmonella dakacs and Salmonella typhi, Salmonella paratyphoid types B and C, Salmonella choleraesuis, Salmonella neoteric No cross-reaction. Immunoblotting showed that the three mAbs bound only to proteins with a Mr 52,000 of S. paratyphi A flagellar antigen. The double mAb sandwich ELISA established by mAb 2E6 and 5D7 was used to detect Salmonella paratyphi A antigen in serum of 13 blood-positive paratyphoid patients. The positive rate was 92-3%.