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慢性乙型肝炎病毒(Hepatitis B Virus,HBV)感染可诱发肝硬化与肝癌,是危害人类健康的重大疾病。基于对HBV感染持续不愈主要是机体免疫功能缺陷所致的认识,提出用“合成免疫”策略重建抗HBV免疫功能,消除感染以预防致命病变的发生。用优化的基因治疗载体微环DNA,在体内表达一套工程抗体,模拟急性HBV感染康复时机体的免疫反应,由单克隆抗体中和病毒,双靶向抗体将非特异的T淋巴细胞转化为抗HBV的T淋巴细胞,杀死HBV感染的肝细胞,两者协同达到根治慢性HBV感染的目的。微环DNA安全、经济,可建立一个安全、高效、可负担的抗HBV免疫体系,有效消除HBV的危害。
Chronic hepatitis B virus (HBV) infection can induce cirrhosis and liver cancer, which is a serious disease endangering human health. Based on the recognition of the uninterrupted HBV infection, which is mainly caused by the defects of immune function, this paper proposes to reconstruct the anti-HBV immune function and eliminate the infection so as to prevent the fatal disease by using the strategy of “synthetic immunization”. An optimized gene therapy vector of minicircle DNA is used to express a set of engineered antibodies in vivo to simulate the immune response of the body during the recovery of acute HBV infection. The virus is neutralized by monoclonal antibodies and the non-specific T lymphocytes are transformed into Anti-HBV T lymphocytes, kill HBV-infected liver cells, the two together to achieve the goal of eradicating chronic HBV infection. Micro-ring DNA safe and economical, can establish a safe, efficient and affordable anti-HBV immune system, effectively eliminate the hazards of HBV.