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目的 :探讨bFGF的反义寡核苷酸对大鼠实验性、外伤性白内障中晶状体上皮细胞增殖和迁移的作用。方法用注射器经角膜将大鼠双眼晶状体的前囊膜刺破并刺入皮质中 ,造成外伤性白内障。然后将 12只动物分为 3组 ,前房分别注射生理盐水、0 .1%反义寡核苷酸和 0 .1%正义寡核苷酸 ,在第 3、7、10和 14天取出眼球 ,用 10 %甲醛溶液固定 ,行病理切片、HE染色 ,在显微镜下观察晶状体上皮细胞的增殖、迁移及赤道部细胞和细胞空泡。结果 :晶状体的组织学显示 ,反义寡核苷酸在第 3、7和 10天在抑制晶状体上皮细胞增殖及迁移方面的作用强于生理盐水及正义寡核苷酸。在赤道部细胞活跃方面 :生理盐水对照组存在 3天 ,正义寡核苷酸存在 2天 ,而反义寡核苷酸没有。在细胞空泡方面 :生理盐水对照组有 2天存在 ,而正义寡核苷酸和反义寡核苷酸组则不存在。统计学分析显示 ,反义寡核苷酸组在抑制晶状体上皮细胞增殖方面的作用显著强于生理盐水组 (P =0 .0 10 4 )和正义寡核苷酸组 (P =0 .0 4 99) ,在抑制晶状体上皮细胞向后囊迁移方面显著强于生理盐水组 (P =0 .0 0 11) ,而与正义寡核苷酸组间差异无显著性意义 (P =0 .0 830 )。在赤道部细胞活跃 (P =0 .4 317)和细胞空泡方面 (P =0 .6 188) ,三组间差异无显著性意义。结论 :?
Objective: To investigate the effect of bFGF antisense oligonucleotide on the proliferation and migration of lens epithelial cells in experimental and traumatic cataract rats. Methods The anterior capsule of the bilateral lens of the rat was punctured with a syringe through the cornea and pierced into the cortex, causing traumatic cataract. Then, 12 animals were divided into 3 groups. The anterior chamber was injected with normal saline, 0.1% antisense oligonucleotide and 0.1% of sense oligonucleotide respectively, and the eye was removed on days 3, 7, 10 and 14 , Fixed with 10% formaldehyde solution, pathological sections, HE staining, observed under a microscope lens epithelial cells proliferation, migration and equatorial cells and cell vacuoles. RESULTS: Histology of the lens showed that antisense oligonucleotides performed stronger inhibitory effects on lens epithelial cell proliferation and migration on days 3, 7 and 10 than saline and sense oligonucleotides. In the equatorial cell activity: saline control group for three days, the existence of sense oligonucleotide for two days, while the antisense oligonucleotide did not. In terms of cell vacuolization: saline control group for two days, while the sense oligonucleotide and antisense oligonucleotide group does not exist. Statistical analysis showed that the effect of antisense oligonucleotide group on the inhibition of lens epithelial cell proliferation was significantly stronger than that of the saline group (P = 0.0104) and the sense oligonucleotide group (P = 0.04 99) was significantly higher than that of the saline group (P = 0.010) in inhibiting the migration of lens epithelial cells to the posterior capsule, but no significant difference with the sense oligonucleotide group (P = 0.030 830 ). In the equatorial cells were active (P = 0.417) and cell vacuoles (P = 0.688), no significant difference between the three groups. in conclusion :?