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建立了一种25%可湿性粉剂的高效液相色谱分析方法,采用反相高效液相色谱法,使用C8柱和紫外检测器,以V(乙腈):V(水)(0.5%冰乙酸)=70:30为流动相,流速为0.8ml/min,在238nm波长下,对西草净进行外标法定量分析。西草净标准偏差为0.10,变异系数为0.38%,平均回收率为99.69%,线性相关系数为0.9996。该方法准确度和精密度均能满足要求,线性关系良好,具有分离效果好,简便、快捷、准确的优点,是一种较为理想的分析方法。
A high performance liquid chromatographic method for the determination of 25% wettable powder was established by reversed-phase high performance liquid chromatography (HPLC) using C8 column and UV detector with V (acetonitrile): V (water) = 70: 30 as the mobile phase at a flow rate of 0.8ml / min. The standard deviation of net grass was 0.10, the coefficient of variation was 0.38%, the average recovery was 99.69% and the linear correlation coefficient was 0.9996. The accuracy and precision of the method can meet the requirements, the linear relationship is good, with the separation effect is good, simple, fast and accurate, is an ideal method of analysis.