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Ca2+是非生物胁迫信号转导途径中的重要信号分子,植物类钙调磷酸酶B亚基蛋白(CBL,calcineurin B-like proteins)是一类重要的钙信号受体蛋白,主要通过与其他蛋白的特异结合传递信号,使植物形成对非生物胁迫的响应。本实验室已经获得大豆Gm CBL1基因,功能鉴定显示Gm CBL1增强了转基因拟南芥对非生物胁迫的耐性。为了进一步研究Gm CBL1的作用机理,本研究构建诱饵载体p GBKT7::Gm CBL1,利用酵母双杂交技术筛选大豆Gm CBL1的互作蛋白。通过对筛选获得的106个蛋白基因测序和Blast比对分析,并根据其可能的生理功能对这些候选蛋白归类,整理得到4类蛋白:能量代谢相关蛋白、修饰蛋白、防御蛋白、钙信号转导相关蛋白。筛选得到候选蛋白的功能预测初步表明,大豆Gm CBL1参与多条信号途径,为进一步研究探索大豆CBL介导的抗逆信号转导途径奠定了基础。
Ca2 + is an important signaling molecule in abiotic stress signal transduction pathway. Plant calcineurin B-like protein is a kind of important calcium signaling receptor protein mainly through binding with other proteins Signaling specifically binds the plant to respond to abiotic stresses. Our laboratory has obtained soybean Gm CBL1 gene, and its functional identification showed that Gm CBL1 enhanced the tolerance of transgenic Arabidopsis to abiotic stress. In order to further study the mechanism of action of Gm CBL1, bait vector p GBKT7 :: Gm CBL1 was constructed and the interaction protein of soybean Gm CBL1 was screened by yeast two-hybrid technique. Through the analysis of 106 protein gene sequences and Blast alignments obtained from the screening, and according to their possible physiological functions, these candidate proteins are classified and sorted into four types of proteins: energy metabolism-related proteins, modified proteins, defensive proteins, calcium signaling Guide related proteins. The functional prediction of the candidate protein by screening initially showed that soybean Gm CBL1 participates in multiple signaling pathways, which lays the foundation for further exploration of CBL-mediated anti-retrograde signaling pathways in soybean.