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目的探讨藜芦(Veratrum nigrum)提取物对DPPH自由基清除作用和对人体肺癌细胞系PC9细胞增殖的影响,比较藜芦的水提物与醇提物的作用差异。方法 DPPH自由基清除法和CCK-8细胞毒性检测法。结果藜芦水提物和醇提物在10mg/ml时体外DPPH清除率分别为52.3%和42.8%。在体外培养的条件下能明显抑制PC9细胞的增殖,并且呈浓度依赖性,而水提物只在高浓度时有抑制作用。结论藜芦提取物具有DPPH自由基清除作用,并且对PC9细胞的体外增殖有明显的抑制作用。
OBJECTIVE: To investigate the effects of Veratrum nigrum extract on DPPH free radical scavenging and the proliferation of human lung cancer cell line PC9 cells, and to compare the effects of veratrum nigrum extract with alcohol extract. Methods DPPH free radical scavenging assay and CCK-8 cytotoxicity assay. Results In vitro DPPH clearance rates of veratridine extracts and ethanol extracts were 52.3% and 42.8% at 10 mg / ml, respectively. In vitro culture conditions can significantly inhibit the proliferation of PC9 cells in a concentration-dependent manner, while the water extract inhibited only at high concentrations. Conclusion Veratrum extract has the DPPH free radical scavenging effect and significantly inhibits the proliferation of PC9 cells in vitro.