白细胞介素2(IL-2)不仅能增强T淋巴细胞介导的免疫反应,还可促进T淋巴细胞的增殖,对其它细胞的作用如何尚不清楚。为此采用体外细胞培养的方法研究了重组IL-2对人体二倍体成纤维细胞的刺激作用。成纤维细胞株的制备是将新鲜人体皮肤用胰蛋白酶消化,使真皮与表皮分离。再将真皮部分加入MEM培养液中制成细胞悬液。取出1.0×10~6细胞移至60mm×15mm培养皿中培养,24小时后 Interleukin 2 (IL-2) not only enhances the T lymphocyte-mediated immune response, but also promotes the proliferation of T lymphocytes, but the effect on other cells is unclear. To this end, the method of in vitro cell culture was used to study the stimulatory effect of recombinant IL-2 on human diploid fibroblasts. Fibroblast strains were prepared by trypsinizing fresh human skin to separate the dermis from the epidermis. Then part of the dermis was added to the MEM medium to prepare a cell suspension. Remove 1.0 × 10 6 cells were transferred to 60mm × 15mm Petri dishes culture, 24 hours later