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目的:探讨缺氧对人食管癌细胞Eca109增殖及凋亡的影响及其作用机制,为食管癌的诊断和治疗提供新的思路和路径。方法:以终浓度为250μmol/L氯化钴模拟缺氧环境,将人食管癌细胞Eca109于常氧及缺氧条件下分别培养12 h、24 h、36 h、48 h,采用倒置相差显微镜观察细胞生长情况,MTT法检测细胞增殖情况,利用细胞活力分析仪NC-3000检测各组细胞凋亡情况。结果:相对于常氧对照组而言,缺氧后各组Eca109细胞增殖减弱,凋亡细胞比率明显升高,其中以缺氧24 h的凋亡细胞比率最高,缺氧36 h的凋亡细胞比率较缺氧24 h时有所回落。结论:缺氧可以抑制人食管癌细胞Eca109的增殖并诱导其凋亡。
Objective: To investigate the effect of hypoxia on the proliferation and apoptosis of human esophageal cancer cell line Eca109 and its mechanism of action, and to provide new ideas and approaches for the diagnosis and treatment of esophageal cancer. Methods: Human esophageal carcinoma cell line Eca109 was cultured in hypoxia environment at a concentration of 250 μmol / L for 12 h, 24 h, 36 h and 48 h under normoxia and hypoxia, respectively. Cell growth, MTT assay cell proliferation, cell viability analyzer NC-3000 detection of apoptosis in each group. RESULTS: Compared with normoxia control group, the proliferation of Eca109 cells in each group was weakened and the percentage of apoptotic cells was significantly increased after hypoxia. The apoptotic cells were the highest in 24 h hypoxia and the apoptotic cells in 36 h hypoxia Compared with the rate of 24 h hypoxia has come down. Conclusion: Hypoxia can inhibit the proliferation and induce the apoptosis of human esophageal carcinoma cell line Eca109.