论文部分内容阅读
目的 检测骨桥蛋白mRNA在大肠癌及其肝转移灶中的表达差异和蛋白定位 ,并探讨其在大肠癌肝转移发生发展中的意义。方法 提取 4 4例大肠癌及癌旁正常组织及 2 0例大肠癌肝转移组织RNA ,采用实时荧光定量PCR(FQ PCR)法检测其中骨桥蛋白mRNA的表达。用免疫组织化学法行骨桥蛋白的组织学检测。根据FQ PCR以CT 值为标准 ,所得数值与实际表达量对数值呈反比的原理 ,以骨桥蛋白CT GAPDHCT 的比值来评价不同组织骨桥蛋白的表达。结果 骨桥蛋白mRNA表达在大肠癌肝转移灶中表达最高 (1 0 7± 0 10 ) ,在原发大肠癌组织中次之 (1 15± 0 14 ) ,而在癌旁正常大肠组织中表达最低 (1 32± 0 18) ;统计分析差异有显著意义 (t=5 81,P =0 0 0 0及t=2 .12 ,P =0 .0 38)。免疫组织化学结果表明 ,骨桥蛋白定位于大肠癌细胞及肝转移灶旁正常肝细胞的细胞质。结论 骨桥蛋白mRNA在大肠癌中的表达高于正常组织 ,而在大肠癌肝转移组织中表达更高 ,骨桥蛋白定位于大肠癌细胞质。这提示骨桥蛋白与大肠癌的浸润转移有关 ,可作为大肠癌肝转移的预测指标之一。
Objective To detect the expression difference and protein localization of osteopontin mRNA in colorectal cancer and its liver metastases and to explore the significance of osteopontin mRNA in the development and progression of hepatic metastases in colorectal cancer. Methods RNA was extracted from 44 cases of colorectal cancer and adjacent normal tissues and 20 cases of colorectal cancer liver metastases. The expression of osteopontin mRNA was detected by real-time fluorescence quantitative PCR (FQ PCR). Histological examination of osteopontin using immunohistochemistry. According to the principle of FQ PCR using CT value as a standard, the obtained value is inversely proportional to the actual expression amount, and the osteopontin expression in different tissues is evaluated by the ratio of osteopontin CT GAPDHCT. Results The mRNA expression of osteopontin was the highest (107 ± 0 10) in colorectal cancer liver metastases, followed by (1 15 ± 0 14) in primary colorectal cancer tissues and adjacent normal colorectal tissues The difference was statistically significant (t = 5 81, P = 0 0 0 0 and t = 2.12, P = 0. 38). Immunohistochemical results showed that osteopontin localized in the cytoplasm of colorectal cancer cells and normal liver cells beside liver metastases. Conclusion The expression of osteopontin mRNA in colorectal cancer is higher than that in normal tissues, but higher in colorectal cancer liver metastasis. Osteopontin is localized in the cytoplasm of colorectal cancer. This suggests that osteopontin is associated with invasion and metastasis of colorectal cancer and may be used as one of the predictors of liver metastasis of colorectal cancer.