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目的用彗星试验检测接触抗癌药物护士的DNA损伤。方法选择16名职业性接触抗癌药物的护士为暴露组,平均暴露工龄为5.6年,平均每天配药7.8份。另选16名从未接触过抗癌药物的护校学生为对照组。用彗星试验检测两组对象外周血淋巴细胞DNA的迁移情况。结果暴露组平均彗星长度为46.27μm,明显高于对照组(26.78μm),差异有显著性(P<0.01)。暴露组平均长尾核的百分率为64.83%,明显高于对照组的4.87%,差异有显著性(P<0.01)。结论暴露组的护士有不同程度的DNA损伤。
Objective To detect DNA damage in nurses exposed to anticancer drugs by comet assay. Methods Sixteen nurses working with occupational exposure to anticancer drugs were selected as the exposure group. The average exposure duration was 5.6 years, with an average of 7.8 servings per day. Another 16 nursing students who had never been exposed to anticancer drugs were selected as the control group. Comet assay was used to detect the DNA migration of peripheral blood lymphocytes in both groups. Results The mean comet length in exposed group was 46.27μm, which was significantly higher than that in control group (26.78μm) (P <0.01). The average percentage of long tail nucleus in the exposed group was 64.83%, which was significantly higher than that in the control group (4.87%, P <0.01). Conclusion Nurses in the exposed group had different degrees of DNA damage.