论文部分内容阅读
目的 为研究肝细胞癌发生发展的机制建立稳定可靠的动物模型。方法 取32只昆明小鼠,采用四氯化碳(CCl_4)/乙醇诱导20周,观察肝癌发生的情况。另取8只作为正常对照。我们采用Northern blot的方法检测了醛缩酶A的mRNA的表达,采用逆转录-聚合酶链反应(RTPCR)的方法检测了甲胎蛋白(AFP)、Cyclin D1、p53、转化生长因子-β1(TGF-β1)、TGF-β1 Ⅰ型受体(TβRI)、Smad4等与肝硬化、肝癌相关的部分基因的mRNA的表达状况。结果 32只昆明小鼠共死亡15只,最后剩余的17只昆明小鼠全部发生肝癌。而正常对照组8只小鼠,其肝脏均未发现明显异常。诱导组小鼠的肝细胞癌类型以中、高分化为主。醛缩酶A、AFP、Cyclin D1、TGF-β1、TβRI、Smad4在肝硬化、肝癌组织中表达不同程度增强,而p53在肝癌组织中表达有所减弱。结论 应用四氯化碳/乙醇诱发的昆明小鼠肝癌,在经历了肝炎、肝硬化的基础上形成,与人肝癌的发生发展过程非常相似。肝脏基因表达的情况也为肝硬化、肝癌的基因治疗提供了一些理论依据。因此,该模型对人类研究肝癌的发生发展及治疗都有实际应用价值,是较理想的肝癌实验动物模型。
Objective To establish a stable and reliable animal model for studying the mechanism of hepatocellular carcinoma (HCC) development. Methods Thirty-two Kunming mice were used to induce hepatocarcinogenesis by using CCl 4 / ethanol for 20 weeks. Another 8 as a normal control. We detected the mRNA expression of aldolase A by Northern blot and detected the expression of AFP, Cyclin D1, p53, transforming growth factor-β1 TGF-β1), TGF-β1 type I receptor (TβRI), Smad4 and other genes related to cirrhosis and liver cancer. Results 32 Kunming mice died a total of 15, the last remaining 17 Kunming mice all occurrence of liver cancer. The normal control group of 8 mice, the liver did not find significant abnormalities. Induction group of hepatocellular carcinoma in mice, mainly in the high differentiation. The expression of aldolase A, AFP, Cyclin D1, TGF-β1, TβRI and Smad4 in cirrhosis and hepatocellular carcinoma tissues increased to some extent, while the expression of p53 decreased in hepatocellular carcinoma tissues. Conclusion The application of carbon tetrachloride / ethanol-induced Kunming mouse hepatocellular carcinoma is formed on the basis of hepatitis and cirrhosis and is very similar to the occurrence and development of human hepatocellular carcinoma. Liver gene expression also provides some theoretical basis for the gene therapy of liver cirrhosis and liver cancer. Therefore, this model has practical value in the study of the occurrence, development and treatment of human liver cancer and is an ideal animal model of liver cancer.