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目的建立槐角丸的HPLC特征图谱,并测定其柚皮苷和新橙皮苷的含量。方法采用RP-HPLC法,色谱柱为Welch AQ C18柱(250 mm×4.6 mm,5μm),以柚皮苷、新橙皮苷、槐角苷、黄芩苷、黄芩素、汉黄芩素为对照,流动相为甲醇-0.05%磷酸,梯度洗脱,检测波长270 nm,流速0.8 mL·min-1,柱温30℃,并进行数字化评价。结果不同来源的槐角丸相似度为0.691~0.998,柚皮苷、新橙皮苷的含量差异较大。结论所用方法简便、准确可行,可评价槐角丸的质量。
Objective To establish HPLC characteristic map of Huaijiao Wan and determine the content of naringin and neohesperidin. Methods The RP-HPLC method was used. The column was Welch AQ C18 column (250 mm × 4.6 mm, 5 μm) with naringin, neohesperidin, sophoridine, baicalin, baicalein and wogonin as controls. The mobile phase consisted of methanol-0.05% phosphoric acid with gradient elution. The detection wavelength was 270 nm, the flow rate was 0.8 mL · min-1 and the column temperature was 30 ℃. Results The similarity of Huaijiao pills from different sources was 0.691 ~ 0.998, and the contents of naringin and neohesperidin were quite different. Conclusion The method used is simple, accurate and feasible, can evaluate the quality of Huaijiao pills.