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Objective: To evaluatetheeffectsof somecytokines,TGF-β 1 anddrugson matrixmetalloproteinases(MMPs)activitiesinculturemediumof arthriticchondrocytesandsynoviocytes.Methods:Thechondrocyteandsynoviocytemono-layersisolatedfromthecartilagesandsynovialfluidsin10kneeOA patientsweretreatedwithIL-1βTGF-β 1 ,TNF-α,di-clofenacacid,dexamethasoneor doxycyclineindividuallyandtogetherfor72h.Zymographywasusedto determinetheac-tivitiesof MMP-2and-9.Results:ThechondrocytemonolayersproducedMMP-2and -9,whilethesynoviocytesonlypro-ducedMMP-2.TheMMP-9activitywasmarkedlyenhancedby IL-1βTNF-αanddiclofenac.IL-1βwasthemosteffective stimulus,andhadsynergisticeffectwithTNF-αor diclifenac.MMP-2activitywas notaffected.Doxcycline,TGF-β 1 and dexamethasonecoulddepresstheactivitiesof MMP-9andMMP-2,and antagonizetheenhancingeffectof IL-1βTNF-αor diclofenac.Conclu sion:IL-1βandTNF-αmayplayimportantrolesdegradingOA cartilage,whileTGF-β 1 anddoxycy-clinemaybe protectivefactors.
Objective: To evaluatetheeffectsof somecytokines, TGF-β 1 anddrugson matrixmetalloproteinases (MMPs) activitiesinculturemediumof arthriticchondrocytesandsynoviocytes.Methods: Thechondrocyteandsynoviocytemono-layersisolatedfromthecartilagesandsynovialfluidsin10kneeOA patientsweretreatedwithIL-1βTGF-β 1, TNF-α, di-clofenacacid, dexamethasoneor doxycyclineindividuallyandtogetherfor72h.Zymographywasusedto determinetheac-tivitiesof MMP-2and-9 . Results: The chondrocytemonolayersproduced MMP-2and-9, whilethesynoviocytes onlypro-duced MMP-2. The MMP-9 activity was markedly enhanced by IL-1βTNF-αand diclofenac.IL-1βwasthemosteffective stimulus, andhadsynergisticeffectwith TNF- α diclifenac.MP- 2activitywas notaffected.Doxcycline, TGF- β 1 and dexamethasonecoulddepresstheactivitiesof MMP-9and MMPs -2, and antagonizetheenhancingeffectof IL-1βTNF-αor diclofenac.Conclu sion: IL-1β and TNF-αmayplayimportantrolesdegradingOA cartilage, while TGF-β 1 anddoxycy-clinemaybe protectivefactors.