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以青藏高原昆仑山垭口不同深度土壤样品为研究对象,研究了可培养细菌数量及多样性.结果表明:可培养细菌数量与多样性在一定程度上均与土壤深度呈负相关关系.可培养细菌数量以表层土壤最多,而细菌多样性最低.基于16S r DNA基因序列分析共发现了6个门,18个属,21种细菌,其中表层土壤Arthrobacter siccitolerans为绝对优势种,比例达95%;冻土区(0~82.15 m)之间不同土样Mycetocola miduiensis菌株所占比例较大;而冻土层以下没有明显的优势菌.冗余分析(RDA)显示:可培养细菌数量主要受土壤有机碳影响,土壤含水量则是影响细菌多样性的主要因素.
Taking the soil samples of different depths from Kunlun Mountain Pass of Qinghai-Tibet Plateau as research objects, the quantity and diversity of culturable bacteria were studied.The results showed that the quantity and diversity of culturable bacteria were negatively correlated with soil depth to a certain degree.Cultivitable bacteria The number of topsoil was the most, while the bacterial diversity was the lowest.A total of sixteen, 18 genera and 21 species of bacteria were found based on 16S rDNA sequence analysis, Arthrobacter siccitolerans was the dominant species in surface soil, with the proportion of 95% Mycetocola miduiensis strains accounted for a large proportion of soil samples (0-82.15 m), while there was no obvious dominant bacteria below the frozen soil layer.RDA analysis showed that the number of culturable bacteria was mainly affected by soil organic carbon Influence, soil moisture is the main factor affecting bacterial diversity.