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我们在对人妊娠5～8周胎盘绒毛和足月胎盘中纤维连接蛋白(FN)进行分离纯化的同时,分析比较了两者的某些生物化学及免疫化学性质。结果:(1)人早期胎盘 FN(epFN)与人足月胎盘FN(tpFN)分子量相似(约500×10~3),是由两个250×10~3 亚基组成,比人血浆FN(pFN)分子量大(约450×10~3)。两者都能与抗人 pFN 抗体反应;(2)epFN、tpFN 及 pFN 都能与抗人羊水 FN 的3个主要功能区单抗发生反应,但 epFN 与抗肝素结合区单抗亲和力大于与抗胶原结合区单抗亲和力,而 tpFN和 pFN 与3种单抗亲和力无明显差异;(3)凝集素结合力实验表明,epFN 与 tpFN 及 pFN 在糖基组成和含量上均有差异;(4)在人早期胎盘绒毛中 FN-结合蛋白的种类和数量都多于足月胎盘。这些结果表明 epFN 可能是人体内一种新的 FN 亚型。
We isolated and purified fibronectin (FN) in human placenta and placenta from 5 to 8 weeks gestation, and compared and analyzed some biochemical and immunochemical properties. Results: (1) The molecular weight of early human placenta FN (epFN) is similar to human full term placenta FN (tpFN) (about 500 × 10-3) and consists of two 250 × 10-3 subunits. Compared with human FN pFN) Large molecular weight (about 450 × 10 ~ 3). Both of them reacted with anti-human pFN antibody; (2) Both epFN, tpFN and pFN reacted with the three major functional domains of anti-human amniotic fluid FN, but the affinity between epFN and anti-heparin binding domain was higher than that of anti- (3) The binding capacity of lectin showed that there were differences in the composition and content of glycans between epFN, tpFN and pFN; (4) In early human placenta, the number and type of FN-binding proteins are more abundant than the full-term placenta. These results suggest that epFN may be a new subtype of FN in humans.