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背景:研究发现脑缺血后多形核白细胞(polymorphonuclearleukocyte,PMNL)与微血管内皮细胞(capillaryendothelialcell,CEC)间的黏附增多,应用抗细胞间黏附分子-1(intercellularadhesionmolecule-1,I-CAM-1)抗体可减轻神经元的缺血性损伤。但是目前还没有直接的证据表明抗-ICAM-1抗体可以抑制PMNL与CEC间的黏附过程。目的:观察脑缺血再灌注后PMNL与CEC间的黏附性变化,探讨应用抗-ICAM-1抗体对脑缺血再灌注后PMNL与CEC间黏附性的影响。设计:完全随机设计。地点和对象:实验地点设在第三军医大学大坪医院神经内科实验室。以成年雄性Wistar大鼠作为实验动物,分为对照组(n=4)、假手术组(n=6)、脑缺血再灌注组(n=10)和治疗组(n=10)。干预:假手术组、脑缺血再灌注组和治疗组大鼠分别于假手术和再灌注4,12,24h后抽取静脉血2～4mL。治疗组大鼠于缺血再灌注后1h静脉注射抗ICAM-1抗体(1mg/kg)。用右旋糖酐沉淀法和Percoll密度梯度离心法分离出PMNL,加入培养的CEC,1h后进行微管吸吮检测。主要观察指标:PMNL与CEC间的黏附力和黏附应力。结果:脑缺血再灌注4h后PMNL和CEC间的黏附力和黏附应力明显升高,并于12h达到高峰。治疗组大鼠在各时间点的黏附力犤4h:(14.3±0.6)N,12h:(16.2±0.8)N,24h:(13.7±0.5)N犦和黏附应力犤4
BACKGROUND: The study found that the adhesion between polymorphonuclear leukocyte (PMNL) and capillary endothelial cell (CEC) increased after focal cerebral ischemia. Intercellular adhesion molecule-1 (I-CAM- Antibodies reduce ischemic damage in neurons. However, there is no direct evidence that anti-ICAM-1 antibody can inhibit the adhesion between PMNL and CEC. Objective: To observe the changes of adhesion between PMNL and CEC after cerebral ischemia-reperfusion and to investigate the effect of anti-ICAM-1 antibody on the adhesion between PMNL and CEC after cerebral ischemia-reperfusion. Design: completely random design. Location and object: The experimental site is located in Daping Hospital Neurology Laboratory, Third Military Medical University. The adult male Wistar rats were divided into control group (n = 4), sham operation group (n = 6), cerebral ischemia reperfusion group (n = 10) and treatment group (n = 10). Intervention: sham operation group, cerebral ischemia reperfusion group and treatment group rats were sham operated and reperfusion 4,12,24h after venous blood drawn 2 ~ 4mL. The rats in the treatment group were injected with anti-ICAM-1 antibody (1mg / kg) intravenously 1h after ischemia-reperfusion. PMNL was isolated by dextran precipitation and Percoll density gradient centrifugation, and cultured in CEC. Microtubule sucking was performed after 1 h. MAIN OUTCOME MEASURES: Adhesion and adhesion stress between PMNL and CEC. Results: The adhesion and adhesive stress between PMNL and CEC increased significantly after cerebral ischemic reperfusion 4h, reaching the peak at 12h. The adhesion of the rats in the treatment group at each time point 犤 4h: (14.3 ± 0.6) N, 12h: (16.2 ± 0.8) N, 24h: (13.7 ± 0.5) N犦 and the adhesion stress 犤 4