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【目的】从感染了青枯病的广藿香植株中分离鉴定青枯菌,并进一步探讨青枯菌的寄主专化性。【方法】采用组织浸泡划线法分离青枯菌;选用青枯菌的特异性引物,对分离菌株进行PCR鉴定;以广藿香、番茄与花生为材料,通过离体叶片接种法和苗期接种法,进行寄主专化性研究。【结果】根据分离菌株在2,3,5-氯化三苯基四氮唑(TTC)培养基上的菌落特征、致病性回接实验和PCR鉴定结果表明,从感染了青枯病的广藿香植株中分离得到的菌株HX6、HX13、HX15与HX17为青枯菌Ralstonia solanacearum。对3种寄主的致病性结果表明,菌株HX15及HX17对广藿香的致病性明显高于番茄和花生;菌株HX6对3种寄主均有较强的致病性。番茄青枯菌GIM1.70对广藿香仅有弱致病性。【结论】从感染了青枯病的广藿香中分离鉴定了4个青枯菌菌株,菌株HX15及HX17的寄主专化性较强,菌株HX6的寄主专化性不明显。
【Objective】 The objective of this study was to isolate and identify R. solanacearum from the patchouli plants infected with bacterial wilt, and to further explore the host specificity of R. solanacearum. 【Method】 Scutellaria oryzae was isolated by tissue immersion and scribing method. Specific primers of R. solanacearum were selected for PCR identification of isolates. Patchouli, tomato and peanut were used as materials, and in vitro leaf inoculation and seedling stage Inoculation method for host specificity studies. 【Result】 According to the colony characteristics of the isolated strains in 2,3,5-triphenyltetrazolium chloride (TTC) medium, pathogenic back-linking experiments and PCR identification showed that from the bacterial infection of bacterial wilt The strains HX6, HX13, HX15 and HX17 isolated from the patchouli plants are Ralstonia solanacearum. The pathogenicity of the three hosts showed that the pathogenicity of the strains HX15 and HX17 to Patchouli was significantly higher than that of tomato and peanut; the strain HX6 had strong pathogenicity to all three hosts. R. solanacearum GIM1.70 is only weakly pathogenic to patchouli. 【Conclusion】 Four strains of R. solanacearum were isolated and identified from the patchouli infected with bacterial wilt. The host specificity of the strains HX15 and HX17 was stronger, and the host specificity of the strain HX6 was not obvious.