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目的 筛选参入及调控阴茎硬结症的相关基因 ,探讨其发病机理及针对相关基因治疗的应答作用。方法 用基因芯片、细胞生物学和分子生物学技术对自行构建的三个阴茎硬结症细胞系进行研究 ,用TGF β1治疗后观察相关基因表达差异。 结果 MCP 1是参与阴茎硬结症病理过程的关键下游基因 ,在硬结及其周围组织构建的细胞系有高表达 (P <0 0 5 ) ,TGF β1治疗后明显上调其表达水平 (P <0 0 5 )。结论 MCP 1在阴茎硬结症的发病过程中起重要作用 ,是某些基因调控的下游基因 ,以此为靶基因的治疗将提高疗效、减少并发症。该研究为进一步明确阴茎硬结症的病因及发病机理和探索中西医结合治疗提供了分子水平的依据
Objective To screen the genes involved in the regulation and treatment of penile sclerosis and to explore its pathogenesis and response to the treatment of related genes. Methods Three penile indurative cell lines constructed by ourselves were studied by gene chip, cell biology and molecular biology techniques. The expression of related genes was observed after treatment with TGF β1. Results MCP 1 was the key downstream gene involved in the pathological process of penile sclerosis. The expression of MCP 1 was significantly up-regulated in the cell lines established by induration and surrounding tissues (P <0 05) 5). Conclusions MCP 1 plays an important role in the pathogenesis of penile sclerosis and is a downstream gene regulated by some genes. As a target gene therapy, MCP 1 will improve the curative effect and reduce the complications. The study provides the molecular basis for further clarifying the etiology and pathogenesis of penile sclerosis and exploring the combination of TCM and Western medicine