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背景:谷氨酸是感觉传入纤维的兴奋性递质,其受体广泛存在于中枢和外周神经系统,在脊髓背角神经元上有特异性分布,介导外周伤害性信息的传递。目的:观察脑室注射谷氨酸对大鼠中枢神经系统丘脑束旁核(PF)痛兴奋神经元(PEN)电生理学的影响。设计:分组对照实验研究。地点和对象:哈尔滨医科大学生理教研室完成,对象为Wistar大鼠30只,体质量240～280g,雌雄不限,由哈尔滨医科大学附属第二医院动物学部提供。干预:实验分4组:①对照组(n=8)。②谷氨酸组(n=18)。③谷氨酸+生理盐水组(n=8)。④谷氨酸+MK-801组(n=10)。分别于脑室注射相应液体。以电脉冲刺激右侧坐骨神经作为伤害性疼痛刺激,用玻璃微电极细胞外记录中枢神经元电活动的变化。主要观察指标:伤害性刺激对PEN电活动的影响,谷氨酸对丘脑PF的PEN电活动的影响,核团注射MK-801阻断谷氨酸对PEN放电的加强作用。结果:①伤害性刺激使大鼠丘脑PF的PEN诱发放电频率增加,刺激前(对照组)放电频率为(2.45±0.89)Hz,刺激后放电频率为(20.34±3.25)Hz,差异有非常显著性意义(t=8.12,P<0.01)。②脑室注射谷氨酸(150mg/L)加强PEN的电活动,使PEN放电频率的净增值增加,潜伏期缩短,注射谷氨酸后8～20min的各项指标分别与对照组同期数据相比,差异有非常显著性意义(t=5.05～7.7
BACKGROUND: Glutamate is an excitatory neurotransmitter for sensory afferent fibers. Its receptors are widely distributed in the central and peripheral nervous systems and are specifically distributed in the dorsal horn neurons of the spinal cord to mediate the transmission of peripheral nociceptive information. Objective: To observe the effects of intracerebroventricular injection of glutamate on the electrophysiology of paraventricular nucleus (PF) pain excitatory neurons (PEN) in rat central nervous system. Design: Group Controlled Experimental Study. Location and object: Department of Physiology, Harbin Medical University, completed 30 Wistar rats, body weight 240 ~ 280g, male or female, provided by the Department of Zoology, the Second Affiliated Hospital of Harbin Medical University. Intervention: The experiment was divided into 4 groups: ① control group (n = 8). ② glutamate group (n = 18). ③ glutamate + saline group (n = 8). ④ glutamate + MK-801 group (n = 10). Respectively in the intraventricular injection of the corresponding liquid. The right sciatic nerve was stimulated by electrical pulse as a nociceptive stimulus. The changes of electrical activity of central neurons were recorded extracellularly by glass microelectrode. MAIN OUTCOME MEASURES: Effects of nociceptive stimuli on electrical activity of PEN, effects of glutamic acid on PEN electrical activity of thalamic PF, and nuclear bolus injection of MK-801 to block the potentiation of PEN discharge. Results: (1) The nociceptive stimulus increased the frequency of PEN-induced discharge in thalamus of PF rats. The frequency of discharge was (2.45 ± 0.89) Hz before stimulation (20.34 ± 3.25) Hz, and the difference was significant Sexual significance (t = 8.12, P <0.01). (2) The intracerebroventricular injection of glutamate (150mg / L) enhanced the electrical activity of PEN, so that the net increase of PEN discharge frequency increases, the incubation period is shortened. The indicators of 8 ~ 20min after glutamate injection compared with the same period of the control group data, The difference is very significant (t = 5.05 ~ 7.7