目的:建立模拟人类感染幽门螺杆菌的小鼠动物模型。方法:选用来自复合性溃疡患者的幽门螺杆菌临床新鲜分离株,以109 CFU·ml1 的菌量按Marta 等报道的方法接种Balb/c 小鼠,最后1 次接种后5 d、2 周、3 周、4 周分别进行检测。结果:接种幽门螺杆菌的实验小鼠胃粘膜均分离出幽门螺杆菌,病理切片显示实验小鼠胃粘膜有不同程度的炎性细胞浸润。结论:选用幽门螺杆菌临床新鲜分离株,按一定方法给小鼠接种,可导致小鼠发生胃炎 Objective: To establish a mouse model of human infection with Helicobacter pylori. Methods: Fresh clinical isolates of Helicobacter pylori from patients with complex ulcer were selected to inoculate Balb / c mice with the dose of 109 CFU ml -1 according to the method reported by Marta et al. After the last vaccination, 5 days and 2 weeks , 3 weeks, 4 weeks were tested. Results: Helicobacter pylori was isolated from the gastric mucosa of mice inoculated with H. pylori. The pathological sections showed that gastric mucosa of experimental mice had different degrees of inflammatory cell infiltration. Conclusion: The selection of fresh clinical isolates of Helicobacter pylori, according to a certain method of vaccination of mice, can cause gastritis in mice