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用异硫氰酸胍方法提取紫花苜蓿超级阿菠萝(Apollo Supreme)植株嫩叶和幼根的总RNA;采用RT-PCR的方法获得紫花苜蓿超级阿菠萝抗旱相关促分裂原活化蛋白激酶MAPK基因中MMK4基因的cDNA序列全长。cDNA序列测定表明:我们克隆到的MMK4基因的cDNA序列全长1116bp,由此推导的氨基酸序列与GeneBank上MMK4(X82270)基因的cDNA序列所编码的氨基酸序列完全相同,但其中有14个氨基酸的密码子有所不同。
The total RNA was extracted from young leaves and young roots of Medicago sativa by guanidine isothiocyanate method. The expression of drought-associated mitogen-activated protein kinase MAPK genes in Medicago sativa Full length cDNA sequence of MMK4 gene. The results of cDNA sequence analysis showed that the cDNA sequence of MMK4 cloned was 1116bp in length. The deduced amino acid sequence of MMK4 gene was exactly the same as that encoded by the cDNA sequence of MMK4 (X82270) on GeneBank, but the 14 amino acids The codons are different.