随着我国农村沼气事业的发展,它的综合效益日益显著,广大群众取得了经济、生态及社会效益。粪便是沼气发酵的原料,又是农业生产的优质有机肥料,也是造成土壤及水源生物污染的主要来源。而土壤是微生物在自然界的最大贮藏所,用未经彻底无害化处理的人畜便施肥,必须造成土壤的生物污染。粪便进入沼气池经一定时间的厌氧发酵是杀灭各种致病菌及寄生虫卵的有效措施。目前,国际上均以大肠杆菌做为衡量食物、水及环境受粪便污染程度。大肠菌群细菌基本上包括全部需氧的革兰氏阴性杆菌,因此将大肠菌群作中粪便污染的指示菌是比较合适 With the development of China’s rural biogas industry, its comprehensive benefits have become increasingly evident. The masses have made economic, ecological and social benefits. Excrement is the raw material for biogas fermentation and is also a high-quality organic fertilizer for agricultural production. It is also the main source of biological pollution of soil and water. However, soil is the largest repository of microorganisms in nature. Fertilization by humans and animals that have not been completely detoxified must result in biological contamination of the soil. Stool into the digester after a certain period of anaerobic fermentation is to kill a variety of pathogenic bacteria and parasite eggs effective measures. At present, Escherichia coli as the measure of food, water and the environment by the degree of fecal contamination. Coliform bacteria basically include all aerobic Gram-negative bacilli, so it is more appropriate to use coliform bacteria as a marker for faecal contamination