目的探讨肝癌及肝硬化患者中ＨＧＶ的感染状态及ＮＳ３区部分基因的酶切分析。采用ＲＴＰＣＲ技术检测ＨＧＶＲＮＡ，酶切分析比较中国株与美国ＧＢＶ－Ｃ株两种基因型的酶切位点。结果８１例肝癌样品中９例ＨＧＶＲＮＡ阳性（１１．１１％），２８例肝硬化中，３例ＨＧＶＲＮＡ阳性（１０．７％）。酶切位点分析结果表明，中国ＨＧＶ感染患者在ＧＢＶ－Ｃ４３５１位存在ＴＳＰ５０９Ｉ（ＡＡ↓ＴＴ）切点，美国和日本发表的１１株ＧＢＶ－Ｃ和１株ＨＧＶ中仅２株存在此切点。结论酶切分析结果提示ＧＢＶ－Ｃ株与中国ＨＧＶ感染株，可能不属于同一基因型 Objective To investigate the infection status of HGV and the restriction analysis of some NS3 genes in patients with liver cancer and cirrhosis. The RTPCR technique was used to detect HGV RNA. The restriction enzyme digestion analysis was used to compare the restriction sites of two genotypes of Chinese and American GBV-C strains. Results Of the 81 samples of liver cancer, 9 were HGV RNA positive (11.11%). Of the 28 patients with liver cirrhosis, 3 were HGV RNA positive (10.7%). The results of restriction enzyme analysis showed that there was TSP509I (AA ↓ TT) at GBV-C4351 in Chinese patients with HGV infection, and only 2 out of 11 GBV-C strains and 1 HGV strain in the United States and Japan had this cut-off point. Conclusions The result of enzyme digestion analysis suggested that GBV-C strain and Chinese HGV infection strain may not belong to the same genotype