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作者用雄性SPrague-Dawley大鼠,体重130±5g,分成三组,每组九只,一组每日皮下注射咖啡因2.5mg/100g体重(按照代谢体重换算相当于70kg体重的人每日吃咖啡340mg、约4杯咖啡),另一组注射咖啡因10mg/100g体重(相当于成人吃1360mg,约16杯咖啡)。对照组注射生理盐水。三组均喂同一饲料,按国家规定的方法饲养,饮食中VitD严格控制。2周后分别测定每只实验动物的尿、粪,血清和饮食中的钙、镁、钾、钠、磷、锌、铜。钙的测定用放射性Ca~(45)作标记,液体闪烁计数器测定。尿、粪、清和饮食中的其他矿物质用马福炉灰化后,原子吸收分光光度法测定。数据用Mann-Whitney检验法统计处理(即秩和检验的方法之一)。
The authors used male SPrague-Dawley rats weighing 130 ± 5 g and divided them into three groups of nine with a daily subcutaneous injection of caffeine 2.5 mg per 100 g of body weight (equivalent to 70 kg of body weight calculated on a metabolic basis, daily eaten About 340 cups of coffee, about 4 cups of coffee) and another group of 10 mg / 100 g of caffeine (equivalent to 1360 mg for adults and about 16 cups for coffee). The control group was injected with saline. The three groups were fed the same feed, according to the method of feeding the country, the diet VitD strict control. After 2 weeks, urine, feces, serum and dietary calcium, magnesium, potassium, sodium, phosphorus, zinc and copper in each experimental animal were measured respectively. Calcium was determined by radioactive Ca ~ (45) labeling and liquid scintillation counter. Urine, excrement, Qing and other minerals in the diet were ashed with a muffle furnace and determined by atomic absorption spectrophotometry. Data were statistically processed using the Mann-Whitney test (ie, one of the methods of rank sum test).