应用杂交瘤单克隆抗体技术制备10株分泌抗牙鲆免疫球蛋白单克隆抗体的杂交瘤细胞。利用单抗2H4,通过间接酶联免疫法(ELISA)对牙鲆血清中抗淋巴囊肿病毒(LCDV)特异性抗体进行了检测。结果表明:无外观症状的牙鲆血清中特异性抗体水平很低(平均OD值为0.092),个别鱼体偏高,证明已感染LCDV,处于潜伏期;患淋巴囊肿病且症状显现的牙鲆血清中特异性抗体水平升高(平均OD值为0.165),患淋巴囊肿病后处于恢复期的牙鲆抗体水平最高(平均OD值为0.231);健康牙鲆接种LCDV灭活疫苗后,特异性抗体水平显著升高。 Ten hybridoma cells secreting anti-Japanese flounder immunoglobulin monoclonal antibody were prepared by hybridoma monoclonal antibody technology. Antibody against lymphocystis disease virus (LCDV) was detected by indirect enzyme-linked immunosorbent assay (ELISA) in monoclonal antibody 2H4. The results showed that the serum level of specific antibodies in flounder with no appearance symptom was very low (mean OD was 0.092). Individual fish were found to be too high to show that they had been infected with LCDV and had latent period. The flounder serum with symptoms of lymphocystis disease (Mean OD was 0.165). The antibody level of flounder was the highest in the recovery period after lymphatic cyst (average OD value was 0.231). After vaccinating the healthy flounder with LCDV inactivated vaccine, the specific antibody Significantly increased levels.