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本文报道用原位缺口翻译法,研究男用节育药醋酸棉酚和雷公藤总甙(GTW)对C3H-10 T 1/2小鼠成纤维细胞DNA的作用。结果表明,在较高浓度的醋酸棉酚或GTW(2～3μg/ml)作用4小时,细胞核中显示的银颗粒较多,如阳性对照组的N-甲基-N′-硝基-亚硝基胍(MNNG)组;在中等浓度的(0.5～1μg/ml)作用下,银颗粒显著地少得多;在较低浓度的醋酸棉酚或GTW作用下(0.1～0.3μg/ml),细胞核中呈现的银颗粒与阴性对照组相似。与原位缺口翻译实验相对应,集落形成试验表明,在高浓度醋酸棉酚或GTW作用下,细胞失去繁殖的能力;而在中等或较低浓度的药物作用下,仍有较高的集落形成率。这些观察表明,高浓度的醋酸棉酚或GTW对细胞DNA有严重的损伤作用,而在中等浓度的药物作用下,DNA受到某种程度的损伤,但可修复;低浓度的醋酸棉酚或GTW对细胞DNA无明显的损伤作用,本文对这些结果的意义进行了简要的讨论。
This paper reports the use of in situ nick translation method to study the effect of male geniocetate acetate and total triptolide (GTW) on DNA of C3H-10 T 1/2 mouse fibroblasts. The results showed that at high concentrations of gossypol acetic acid or GTW (2 to 3 μg/ml) for 4 hours, there were more silver granules in the nucleus, as in the positive control group N-methyl-N′-nitro-a In the nitroguanidine (MNNG) group, silver particles were significantly less at moderate concentrations (0.5 to 1 μg/ml); at lower concentrations of gossypol acetate or GTW (0.1 to 0.3 μg/ml) The silver particles present in the nucleus are similar to the negative control group. Corresponding to the in situ nick translation experiments, colony formation assays showed that the cells lost the ability to reproduce under the action of high-concentration gossypol acetate or GTW, while there was still a higher colony formation under the action of medium or lower concentrations of drugs. rate. These observations indicate that high concentrations of gossypol acetic acid or GTW have a severe damage to cellular DNA, while under moderate concentrations of the drug DNA is damaged to some extent but repairable; low concentrations of gossypol acetic acid or GTW There is no obvious damage to cellular DNA. This article briefly discusses the significance of these results.