高中生物选修1中的“大肠杆菌的培养和分离”一节,是生物技术实践的基础,具体要求掌握:无菌技术的操作(此乃微生物培养、植物组培等关键所在);了解有关培养基的基础知识,利用液体培养基进行大肠杆菌扩增培养的操作;用固体平面培养基进行大肠杆菌的划线培养和分离,了解涂布分离法;大肠杆菌培养的条件和实验原理。此部分内容与必 The section “Culture and Separation of E. coli” in high school biology elective 1 is the basis for biotechnological practice with specific requirements for mastery of aseptic techniques (this is where microbiological cultures, plant tissue culture, etc.) are located; The basics of culture medium, the use of liquid medium for the expansion of E. coli culture operations; solid flat medium for E. coli culture and separation line to understand the coating separation; Escherichia coli culture conditions and experimental principles. This part of the content and will