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为明确芸薹根肿菌Plasmodiophora brassicae Woron.在其它寄主中是否广泛存在无性短循环及次生游动孢子的侵染致病性,以不结球白菜为寄主培养3批幼苗(G1、G2和G3),用休眠孢子悬浮液接种G1,被侵染的G1接种G2,被侵染的G2接种G3,采用离心管水培法研究其侵染致病性。结果显示,无性短循环研究中,G1、G2和G3根毛均被侵染,除G3并株接种侵染率为33.33%外,其它处理侵染率均在50.00%以上,根毛里有明显的游动孢子囊;次生游动孢子能侵染不结球白菜的皮层组织,致使不结球白菜发病形成明显的肿根;G1、G2和G3水培发病率为20.00%、15.00%和6.00%,砂培发病率为22.50%、18.75%和7.50%;G3肿根病理切片中可观察到休眠孢子。表明芸薹根肿菌侵染不结球白菜时,其生活史中存在无性短循环,次生游动孢子具有侵染致病作用。
In order to clarify whether Plasmodiophora brassicae Woron from Brassica oleracea is widespread in other hosts, and the pathogenicity of infection of secondary zoospores is extensive. Three batches of seedlings (G1, G2 and G3), inoculated with resting spore suspension G1, inoculated G1 inoculated G2, inoculated G2 inoculated G3, the use of centrifugal water culture to study the pathogenicity of its infection. The results showed that in the asexual short cycle study, G1, G2 and G3 root hairs were all infected, with the exception of the G3 strain and the inoculation rate was 33.33%, the other treatment rates were above 50.00% The secondary zoospores could infect the cortical tissues of non-heading Chinese cabbage, resulting in obvious swollen roots in non-heading cabbage. The incidence rates of hydroponics of G1, G2 and G3 were 20.00%, 15.00% and 6.00% , Sand culture incidence of 22.50%, 18.75% and 7.50%; G3 edema pathological sections can be observed in resting spores. Show that Brassica root blight infection of non-heading Chinese cabbage, the existence of asexual short cycle of life cycle, secondary zoospores infection pathogenic role.