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脂肪酸是人体肝细胞中的重要成分。在正常情况下它处于相对稳定状态。而当患肝癌时。这种相对平衡状态会被破坏。使脂肪酸的种类和含量发生变化。本实验应用气相色谱—质谱法检测了人体正常肝及肝细胞癌各30份。30份正常肝样品均测得三癸烷酸(C_(13:0))、肉豆蔻酸(C_(14:0))、棕榈酸(C_(16:0))、硬脂酸(C_(18:0))、十八碳—烯酸(C_(18:1))、十八碳二烯酸(C_(18:2))。29份样品测得十五烷酸(c_(15:0)),28份样品测得十六碳一烯酸(C_(16:1))、二十碳四烯酸(C_(20:4))。30份肝癌样品均未测得十五烷酸(C_(15:0)),7份样品测得 C_(14:0)。明显少于正常肝。其余脂肪酸虽所测得种类无明显差异但含量明显低于正常肝。据此可对肝癌进行病理诊断。本实验得出了人体正常肝组织及肝癌组织脂肪酸的色谱图及质谱图。为在分子水平上对肝癌做病理诊断开辟了一个新的领域。
Fatty acids are an important component of human liver cells. It is relatively stable under normal conditions. And when suffering from liver cancer. This relative balance will be destroyed. Change the type and content of fatty acids. In this experiment, 30 human normal liver and hepatocellular carcinomas were detected by gas chromatography-mass spectrometry. In 30 normal liver samples triterpenic acid (C_(13:0)), myristic acid (C_(14:0)), palmitic acid (C_(16:0)) and stearic acid (C_() were all measured. 18:0)), octadecanoic acid (C18:1), octadecadienoic acid (C18:2). The pentadecanoic acid (c_(15:0)) was measured in 29 samples, and hexadecenoic acid (C_(16:1)) and eicosatetraenoic acid (C_(20:4) were found in 28 samples. )). None of the 30 liver cancer samples were measured for pentadecanoic acid (C_(15:0)), and 7 samples were measured for C_(14:0). Significantly less than normal liver. Although the remaining fatty acids were not significantly different, the content was significantly lower than that of normal liver. According to this, pathological diagnosis of liver cancer can be made. In this experiment, the chromatograms and mass spectra of normal human liver tissue and liver cancer tissues were obtained. To open up a new field for pathological diagnosis of liver cancer at the molecular level.