本文探讨了胰岛素效价的盘电泳测定和酶联免疫吸附测定,并对两种方法的测定结果进行比较。结果表明两种方法的灵敏度均较高,检出限量分别为盘电泳3μg,酶联免疫吸附测定6μu。且一次操作可同时测定数个样品。经适当处理校正后,电泳测定值与酶联免疫吸附测定值基本一致。这两种方法应用于胰岛素的分离纯化过程的活力监控取得了较满意的结果。 This article explored the determination of insulin potency by disk electrophoresis and enzyme-linked immunosorbent assay, and the results of the two methods were compared. The results showed that the sensitivity of the two methods were high, the detection limit were plate electrophoresis 3μg, enzyme-linked immunosorbent assay 6μu. And a single operation can be measured at the same time several samples. After proper treatment and correction, the measured values ​​of electrophoresis and ELISA are basically the same. These two methods used in the separation and purification of insulin vitality monitoring achieved more satisfactory results.