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目的建立甲胺磷的犬灌胃染毒致死模型,观察甲胺磷在犬体内的死后分布规律。方法犬经8倍LD50(7.4mg/kg)剂量甲胺磷灌胃后,观察其中毒症状,死亡后即刻解剖,分别取心、肝、脾、肺、肾、脑、右上肢肌、右下肢肌、胸肌、胃组织、心血、胆汁、玻璃体液和尿液,GC/MS和GC法检测其中甲胺磷含量。结果犬8倍LD50甲胺磷灌胃染毒后20min内出现中毒症状(,53.3±14.1)min死亡。各组织脏器及体液中甲胺磷含量由高到低分别为胃(99.84±0.87)μg/g、脾(46.87±28.67)μg/g、肝(43.82±22.74)μg/g、肾(43.79±29.04)μg/g、心血(35.36±13.98)μg/mL、肺(35.25±18.59)μg/g、尿34.81μg/mL、胸肌(19.23±17.18)μg/g、右上肢(16.92±8.98)μg/g、心(15.09±6.11)μg/g、右下肢(12.83±7.63)μg/g、脑(10.91±4.13)μg/g、胆汁(6.75±1.45)μg/mL、玻璃体液(6.22±4.97)μg/mL。结论甲胺磷在犬体内死后分布不均,胃、脾、肝、肾、心血、肺、尿检材中含量较高,可作为疑似甲胺磷中毒毒物分析的检材。
OBJECTIVE To establish the intragastric model of methamidophos in mice and observe the distribution of methamidophos in dogs after death. Methods Dogs were anesthetized with 8 times the dose of LD50 (7.4mg / kg), and their symptoms were observed immediately after death. Heart, liver, spleen, lung, kidney, brain, right upper limb muscle and right lower limb Muscle, chest muscle, stomach tissue, cardiovascular, biliary, vitreous and urine, GC / MS and GC method for the determination of methamidophos content. Results 8-fold LD50 methamidophos gavage poisoning within 20min after symptoms (53.3 ± 14.1) min death. The levels of methamidophos in organs and body fluids from high to low were 99.84 ± 0.87 μg / g, 46.87 ± 28.67 μg / g and 43.82 ± 22.74 μg / g, respectively (35.25 ± 18.59) μg / g, urine 34.81 μg / mL, breast muscle (19.23 ± 17.18) μg / g, right upper limb (16.92 ± 8.98) μg / g, (15.09 ± 6.11) μg / g, right lower limb (12.83 ± 7.63) μg / g, brain (10.91 ± 4.13) μg / g and bile (6.75 ± 1.45) μg / mL and vitreous fluid (6.22 ± 4.97) μg / mL. Conclusion Methamidophos is unevenly distributed in dogs after ingestion. The content of methamidophos in stomach, spleen, liver, kidney, heart, lungs and urine is high, which can be used as the material for the analysis of methamidophos poisoning.