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本文用免疫细胞化学(ICC)和显微分光光度计测定技术观察正常大鼠及其在肠型放射病(AIRS)时舌内降钙素基因相关肽(CGRP)免疫反应性(IP)神经的变化。结果表明,舌内各组织中均有CGRP-IR神经分布。在AIRS时,CGRP-IP神经发生明显变化,可分为二个时期,照射后24h内为反激期,表现为舌组织中CCRP-IR神经明显肿胀增粗,膨体增长,神经末梢聚集物增多。照射后48—72h为破坏期。其病变特征:照射后48h舌体CGRP-IR神经减少,变细,膨体变小,数目下降,部分CGRP-IR神经显著减少,甚至消失,舌根部除舌肌上的运动终板样结构和味腺内CGRP-IR神经未见明显破坏,其它所有CGPR-IR神经几乎完全断裂成点状。显微分光光度计测量结果,照射后48h及照射后72h与正常相比总吸光度显著降低(P<0.01)。本实验证明在大剂量辐射损伤时,舌内肽能神经也有损伤操作,为AIRS的发病机制,病理损伤治疗提供了参考资料。
In this study, immunocytochemistry (ICC) and microspectrophotometer were used to observe the changes of CGRP immunoreactive (IP) nerve in normal rats and their gut during radiotherapy of intestinal type radiosurgery (AIRS) . The results showed that there was CGRP-IR nerve distribution in each tongue. In AIRS, CGRP-IP nerve changes significantly, can be divided into two periods, within 24h after irradiation as the anti-shock period, manifested as tongue tissue CCRP-IR nerve swelling significantly thicker, enlarged, nerve endings aggregates increase. 48-72h after irradiation as the destruction period. The lesion characteristics: The CGRP-IR nerves of the tongue decreased, thinned and the size of the CGRP-IR neurons decreased 48h after irradiation, and the number decreased. Some CGRP-IR nerves were significantly reduced or even disappeared. No significant damage was observed in the CGRP-IR nerves in the gusta, and almost all other CGPR-IR nerves broke into dots. The results of micro-spectrophotometer showed that the total absorbance decreased significantly at 48h and 72h after irradiation (P <0.01). This experiment proved that in high-dose radiation injury, the tongue nerve can also damage the operation for the pathogenesis of AIRS, pathological injury treatment provides a reference.