人教版高中生物学必修1“探究酵母菌细胞呼吸的方式”实验提到了用溴麝香草酚蓝水溶液检测CO2产生的方法:CO2可使澄清的石灰水变浑浊,也可使溴麝香草酚蓝水溶液由蓝变绿再变黄。多数师生对该方法检测CO2产生的原理很陌生,部分师生用该方法检测CO2产生时发现实验效果不明显,看不到明显的变色过程。笔者认为主要原因是师生对溴麝香草酚蓝的性质及检测原理并不了解,在配制溴麝香草酚蓝水溶液时也没有注意到一些细节,从而导 People’s PEP version of high school biology compulsory 1 “way to explore the yeast cells to breathe” experiment referred to the use of bromothymol blue aqueous solution of CO2 detection method: CO2 can clarify the lime water turbidity, but also bromine musk The aqueous solution of phenol blue from blue to green and then yellow. Most teachers and students of this method is very strange to the principle of detecting CO2 production, some teachers and students using this method to detect CO2 production found that the effect is not obvious, can not see the obvious discoloration process. I think the main reason is that teachers and students do not understand the nature of bromothymol blue and the principle of detection, in the preparation of bromothymol blue aqueous solution also did not notice some details, which guide