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研究了环境因子如K+、Na+、Ca2 +、Mg2 +、Cl- 、SO2 -4 以及蔗糖、pH、氨海水对皱纹盘鲍 (HaliotisdiscusHannai)和太平洋牡蛎 (Creastreagigas)精子运动及受精率的影响。精子在 0 4～ 0 5mol/L的NaCl、KCl溶液中运动能力为 1 0 0 % ,但受精能力较差或完全不受精。在替代海水中无Ca2 +的情况下 ,牡蛎不受精 ,但鲍有较低的受精能力。Mg2 +作用不明显。在 0 5～ 0 6mol/L蔗糖溶液中鲍精子能运动 ,且有较低的受精能力 ,但牡蛎精子完全不能运动和受精。氨海水对精子运动有一定的刺激作用。在 pH为 6 0～8 0时精子运动能力较强 ,但受精的 pH适宜范围为 7 0～ 8 0 ,同时鲍和牡蛎在精子运动和受精方面有明显差异
The effects of environmental factors such as K +, Na +, Ca2 +, Mg2 +, Cl-, SO2-4, sucrose, pH and ammonia concentration on the sperm motility and fertilization rate of Haliotis discus hannai and Pacific oyster (Creastreagigas) were studied. Sperm in 0 4 ~ 0 5mol / L of NaCl, KCl solution kinetic capacity of 100%, but poor fertilization or no fertilization. In the absence of Ca2 + in seawater, oysters are not fertilized, but abalone has lower fertility. Mg2 + role is not obvious. In 0 5 ~ 0 6mol / L sucrose solution, spermatozoa can move and have lower fertilization ability, but oyster sperm can not exercise and fertilize at all. Ammonia sea water on sperm motility have a certain stimulating effect. At pH 60 ~ 80, the ability of sperm movement was stronger, but the pH of fertilization ranged from 70% to 80%, while there was a significant difference between sperm motility and fertilization