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合成了一个新的L-苯丙氨酸邻香草醛席夫碱及邻菲咯啉混配铜(Ⅱ)配合物[Cu(o-van-phe)(phen)]·CH3OH.通过元素分析、红外光谱对其进行了表征.采用X射线单晶衍射测定其晶体结构,结果表明该配合物属于单斜晶系,C2空间群:a=1.15637(17)nm,b=1.8562(2)nm,c=1.34249(18)nm,β=98.385(2)°,V=2.8509(7)nm3,Dc=1.335 g/cm3,Z=4,F(000)=1188,最终的偏差因子为R1=0.0574,wR2=0.1540[Ⅰ>2σ(Ⅰ)].五配位的铜离子形成一个畸变的四方锥配位构型,相邻两主体分子之间通过氢键形成二聚体结构.紫外光谱、荧光光谱和圆二色光谱测定结果表明,配合物与小牛胸腺DNA(CT-DNA)以插入方式相互作用,结合常数Kb=9.697×103 L·mol-1.凝胶电泳研究表明,配合物可将环形质粒pBR322 DNA切割为缺刻型和线型DNA.
A new copper (Ⅱ) complex [Cu (o-van-phe) (phen)) · CH3OH was synthesized by the reaction of a new L-phenylalanine-vanillin Schiff base and phenanthroline. By elemental analysis, The results show that the complex belongs to the monoclinic system. The C2 space group is a = 1.15637 (17) nm, b = 1.8562 (2) nm, The final deviation factor is R1 = 0.0574 (18) nm, β = 98.385 (2) °, V = 2.8509 (7) nm3, Dc = 1.335 g / cm3, Z = 4 and F , wR2 = 0.1540 [I> 2σ (Ⅰ)]. Five copper ions coordinate to form a distorted tetragon pyramid structure, and the two adjacent host molecules form a dimer structure through hydrogen bonding. The results of spectroscopic and circular dichroism spectroscopy showed that the complex interacted with the CT-DNA of calf thymus DNA with the binding constant Kb = 9.697 × 103 L · mol-1. The results of gel electrophoresis showed that the complex The circular plasmid pBR322 DNA was cut into nicked and linear DNA.