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背景:传统观点认为中枢神经组织在发育成熟和损伤后不能再生,但近年来研究证实,人及成年动物神经系统中均存在神经干细胞,只是大部分神经干细胞在体内处于静止状态。神经干细胞对脑梗死损伤的治疗作用,已成为研究的焦点问题。目的:观察脑梗死后内源性神经干细胞的反应过程,探讨内源性神经干细胞在中枢神经系统损伤修复中的作用,为脑梗死损伤后机体自我修复提供理论依据。设计:以实验动物为研究对象,随机、对照的实验研究。单位:北京协和医院神经外科。材料:实验于2003-03/10在中国医学科学院中国协和医科大学北京协和医院神经外科实验室完成。选择健康雄性Wistar大鼠82只,体质量250～300g。方法:用线栓法制作大鼠脑梗死模型,将其分成梗死后1,3,7,14,28d组,每组14只,对照组为假手术组(12只)。免疫组织化学方法动态检测大鼠脑内5-溴脱氧尿苷嘧啶(BrdU)、巢蛋白(Nestin)的表达。主要观察指标:大鼠脑梗死后BrdU,Nestin阳性细胞数的变化。结果:对照组中,海马齿状回及SVZ区存在少量BrdU和Nestin阳性细胞,脑梗死后1d,海马和SVZ区BrdU阳性细胞较对照组显著增加(P<0.05);7d达到高峰(P<0.05);14d后开始下降,但仍高于正常水平(P<0.05);28d后接近正常。并且,梗死侧BrdU和Nestin阳性细胞数明显多于对侧(P<0.05),并且?
Background: The traditional view is that the central nervous system can not regenerate after its maturation and injury. However, studies in recent years confirmed that neural stem cells are present in the nervous systems of both humans and adult animals, but most of the neural stem cells are in a quiescent state in the body. The therapeutic effect of neural stem cells on cerebral infarction has become the focus of research. OBJECTIVE: To observe the reaction process of endogenous neural stem cells after cerebral infarction, to explore the role of endogenous neural stem cells in the repair of central nervous system injury, and to provide a theoretical basis for self-repair of the body after cerebral infarction. Design: The experimental animals as the research object, randomized, controlled experimental study. Unit: Beijing Union Medical College Hospital Department of Neurosurgery. MATERIALS: The experiment was performed at the Neurosurgical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences from March to October in 2003. 82 healthy male Wistar rats were selected and their body weight was 250-300g. Methods: The rat model of cerebral infarction was established by thread occlusion and divided into groups of 1, 3, 7, 14 and 28 days after infarction, with 14 rats in each group. The control group was sham operation group (12 rats). Immunohistochemistry was used to detect the expression of BrdU and Nestin in rat brain. MAIN OUTCOME MEASURES: Changes of BrdU and Nestin positive cells after cerebral infarction in rats. Results: BrdU and Nestin positive cells were found in the hippocampal dentate gyrus and the SVZ area. BrdU positive cells in the hippocampus and SVZ increased significantly (P <0.05) on the 1st day after cerebral infarction compared with the control group, and reached peak on the 7th day (P < 0.05). After 14 days, it began to decline, but still higher than the normal level (P <0.05). After 28 days, it was close to normal. Moreover, the number of BrdU and Nestin positive cells in the infarct side were significantly more than those in the contralateral (P <0.05)