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采集黑龙江省不同地区田间210份大豆检测样品,利用定性PCR技术分析检测其中是否含有转基因成分(CaMV35S启动子、NOS终止子和CP4-EPSPS基因).结果表明,210份样品中均未检测到CaMV35S启动子成分;在13个样品中虽扩增出类似CP4-EPSPS的条带,但对PCR产物的进一步酶切鉴定表明,所有扩增结果为假阳性.利用巢式PCR对样品0x1的进一步分析表明,该样品中不含有转基因大豆(roundupready)成分.对0x1样品用CP4-EPSPS引物扩增得到的片段进行了测序.序列分析表明,该片段与CP4-EPSPS基因的同源性仅有81%,再次证明它不是转基因大豆的CP4-EPSPS基因.
A total of 210 soybean samples from different regions of Heilongjiang province were collected and analyzed by qualitative PCR to detect whether they contained the transgenic components (CaMV35S promoter, NOS terminator and CP4-EPSPS gene) .The results showed that CaMV35S Promoter components were detected in all the samples.Although a band similar to CP4-EPSPS was amplified in 13 samples, further restriction analysis of the PCR products indicated that all amplification results were false positives. Further analysis of sample 0x1 by nested PCR The results showed that there was no roundup ingredient in the sample.The fragment amplified by CP4-EPSPS primer was sequenced for the 0x1 sample.Sequencing analysis showed that the fragment shared 81% homology with the CP4-EPSPS gene, , Again demonstrating that it is not the CP4-EPSPS gene of transgenic soybeans.