论文部分内容阅读
目的研究多聚ADP核糖聚合酶(poly(ADP-ribose)polymerase,PARP)抑制剂3-氨基苯甲酰(3-aminobenzamide,3-AB)对脂多糖(lipopolysaccharide,LPS)诱导的帕金森病(Parkinson’s disease,PD)大鼠的血脑屏障(blood–brain barrier,BBB)及多巴胺能神经元的影响。方法大鼠随机分三组:对照组,LPS组和LPS+3-AB组。用伊文思兰渗透性实验检测血脑屏障通透性;Western blot法检测MMP-9和紧密连接蛋白ZO-1的表达;免疫组化法检测MMP-9在黑质神经元的表达。结果与对照组比较,LPS组黑质内BBB的通透性和MMP-9的表达显著增加,紧密连接蛋白相关蛋白ZO-1的表达和酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性细胞数显著降低。上述作用受到PARP抑制剂3-AB的显著抑制。结论 3-AB通过保护LPS诱导的PD大鼠的BBB进一步保护多巴胺能神经元。
Objective To investigate the effect of 3-aminobenzamide (3-AB), a poly ADP ribose polymerase (PARP) inhibitor, on Parkinson’s disease (LPS) -induced Parkinson’s disease (PDB) on the blood-brain barrier (BBB) and dopaminergic neurons in Parkinson’s disease (PD) rats. Methods Rats were randomly divided into three groups: control group, LPS group and LPS + 3-AB group. The permeability of blood-brain barrier was detected by Evans blue permeation assay. The expression of MMP-9 and tight junction protein ZO-1 was detected by Western blot. The expression of MMP-9 in substantia nigra neurons was detected by immunohistochemistry. Results Compared with the control group, the BBB permeability and the expression of MMP-9 in substantia nigra were significantly increased, the expression of tight junction protein ZO-1 and tyrosine hydroxylase (TH) positive cells The number is significantly lower. This effect was significantly inhibited by PARP inhibitor 3-AB. Conclusion 3-AB protects dopaminergic neurons further by protecting the BBB of LPS-induced PD rats.