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目的:筛选出大血藤酚酸类化学成分的最佳提取工艺。方法:首先建立HPLC测定大血藤中没食子酸、原儿茶酸、绿原酸方法,采用正交试验设计,以提取时间、甲醇体积分数、溶媒用量、提取次数为因素考察大血藤回流提取方法,以没食子酸、原儿茶酸、绿原酸为评价指标,确定最佳提取条件。结果:优选工艺为用10倍量80%甲醇提取3次,每次30 min。结论:采用多指标综合评价法优化大血藤酚酸类化学成分提取工艺合理、可行。
Objective: To screen out the best extraction technology for the chemical constituents of Saponaria. Methods: The HPLC method was established for the determination of gallic acid, protocatechuic acid and chlorogenic acid in Radix et Rhodiomae. The orthogonal design was used to determine the extraction time, the volume fraction of methanol, the amount of solvent and the extraction times Methods, with gallic acid, protocatechuic acid, chlorogenic acid as the evaluation index to determine the best extraction conditions. Results: The preferred process was to extract three times with 10 times the amount of 80% methanol for 30 min each. Conclusion: It is reasonable and feasible to optimize the extraction process of chemical constituents of Saphornoides by multi-index comprehensive evaluation.