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[目的]研究紫穗槐种子中杀菌活性成分。[方法]利用硅胶柱层析,高速逆流色谱法等方法对紫穗槐种子的乙酸乙酯提取物进行分离,并对提取化合物进行杀菌活性测定。[结果]从紫穗槐种子的乙酸乙酯提取物中分离得到7个化合物,分别为5-羟基-7,4’-二甲氧基异黄酮、2’-羟基-4,4’-二甲氧基查耳酮、7,4’-二甲氧基异黄酮、鱼藤酮、11-羟基灰叶素、异灰毛豆酚、异灰叶素。其中鱼藤酮、11-羟基灰叶素、异灰毛豆酚、异灰叶素对黄瓜霜霉活性较好。[结论]紫穗槐中杀菌活性成分主要是鱼藤酮类化合物。
[Objective] The research aimed to study the germicidal active ingredients of Amorpha fruticosa seeds. [Method] The ethyl acetate extracts from Amorpha fruticosa were separated by silica gel column chromatography and high speed countercurrent chromatography. The antibacterial activities of the compounds were determined. [Result] Seven compounds were isolated from the ethyl acetate extract of Amorpha fruticosa L. seeds, which were 5-hydroxy-7,4’-dimethoxyisoflavone, 2’-hydroxy- Methoxy chalcone, 7,4’-dimethoxyisoflavone, rotenone, 11-hydroxy limonin, isoamericol, iso-limonin. Rotenone, 11-hydroxy-limonin, iso-limonin and iso-limonin exhibited good activity against cucumber downy mildew. [Conclusion] The active ingredient of Amorpha fruticosa was mainly rotenone.