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1974年英国Cercek等报告,以荧光偏振技术测定癌患者末梢血淋巴细胞的胞浆基质结构程度(structuredness of Cytoplasmic matrix,简称SCM)的改变可用于肿瘤的诊断,由于方法敏感、结果可靠,这一方法遂引起有关方面的广泛注意。作者等将淋巴细胞加入二醋酸荧光素(FDA)溶液中,FDA为无荧光物质,当透入细胞后即被酯酶水解而释出荧光素,测定唇者的荧光偏振度——荧光素分子空间排列的有序程度——即可间接
In 1974, Cercek et al reported that the change of the cytoplasmic matrix structure (SCM) of peripheral blood lymphocytes in cancer patients by fluorescence polarization can be used in the diagnosis of tumors. Because of the sensitivity of the method and the reliable results, The method has aroused widespread attention. The authors add lymphocytes to a solution of diacetate acetate (FDA), which is a non-fluorescent substance that is hydrolyzed by esterase to release fluorescein when it penetrates into the cell. The fluorescence polarization of the lip is measured - the fluorescein molecule The degree of spatial arrangement of the order - that can be indirect