论文部分内容阅读
目的:在糖尿病肾病早期尿蛋白排泄与肾脏组织学变化相关关系研究的基础上,进一步探讨早期糖尿病肾病肾脏体积肥大的潜在机制。方法:通过STZ诱导糖尿病动物模型,ELISA法测定其24小时尿白蛋白排泄率(UAER),光电镜观察糖尿病刚成模组及1、2、3、4周不同病程组的糖尿病大鼠肾脏组织学改变,用图像分析肾小球基底膜厚度、肾小球面积、肾小球细胞总数、平均细胞面积。结果:不同病程糖尿病鼠肾小球面积、肾小球细胞总数、平均细胞面积、肾小球基底膜厚度随病程延长而增加,但肾小球细胞总数的增加无显著性差异(P>0.05)。相关分析表明,肾小球基底膜厚度与尿白蛋白排泄率呈正相关,肾小球平均细胞面积与肾小球面积呈正相关。结论:肾小球基底膜滤过屏障受损是产生白蛋白尿的直接原因,肾小球细胞肥大与肾小球面积肥大有一定关系。
OBJECTIVE: To further investigate the underlying mechanism of renal hypertrophy in diabetic nephropathy on the basis of previous studies on urinary protein excretion and renal histological changes in patients with diabetic nephropathy. Methods: Animal model of diabetes was induced by STZ. The urinary albumin excretion rate (UAER) was measured by ELISA and the diabetic rats’ kidney tissues were observed by light microscope at different developmental stages of diabetes mellitus Learning to change, the image analysis of glomerular basement membrane thickness, glomerular area, the total number of glomerular cells, the average cell area. Results: The glomerular area, the number of glomerular cells, the average cell area and the thickness of glomerular basement membrane increased with the course of disease in different stages of diabetic rats, but there was no significant difference in the total number of glomerular cells (P> 0.05) . Correlation analysis showed that glomerular basement membrane thickness and urinary albumin excretion rate was positively correlated, glomerular average cell area and glomerular area was positively correlated. Conclusion: The impaired glomerular basement membrane filtration barrier is the direct cause of albuminuria, glomerular hypertrophy and glomerular hypertrophy have a certain relationship.