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目的分析慢性乙型肝炎患者外周血树突状细胞(DC)上Toll样受体3(TLR3)的表达变化及其临床意义。方法随机筛选慢性乙型肝炎(CHB)患者20例,并以10例健康者作对照,分别从外周血分离单个核细胞,体外诱导培养DC。流式细胞仪(FCM)测定未成熟期DC(imDC)与成熟期DC(mDC)的表型;分别应用流式细胞仪和Western印迹法测定imDC与mDC上TLR3的表达率和受体蛋白的表达。结果在健康对照组,mDC表面分子CD80、CD86、组织相容性白细胞抗原(HLA)- DR、CD83的表达率分别为(82.35±8.67)%、(79.61±10.08)%、(92.79±8.48)%和(83.76±5.47)%,imDC表面分子表达率分别为(28.31±8.79)%、(31.17±11.23)%、(27.61±10.28)%和(23.46±11.53)%,各组间比较,差异均有统计学意义(P<0.01);而在CHB组,mDC与imDC表面分子表达率分别为(42.46±9.22)%、(40.72±11.24)%、(48.57±12.51)%、(22.25±11.22)%和(18.57±10.22)%、(24.16±10.46)%、(17.87±10.38)%、(14.28±6.77)%,各组间比较,差异均无统计学意义(P>0.05);健康者组mDC上表面分子的表达率与CHB患者组比较,各组间差异均有统计学意义,而两组受试者imDC上表面分子的表达率比较,差异均无统计学意义。健康对照imDC上TLR3的表达率明显高于mDC(P<0.01),而CHB患者imDC与mDC上TLR3的表达率差异无统计学意义(P>0.05);且CHB患者imDC上TLR3的表达率明显低于健康对照(P<0.01)。结论CHB患者外周血DC上存在TLR3的表达下调,可能是HBV感染慢性化的重要原因。
Objective To analyze the changes of Toll like receptor 3 (TLR3) in peripheral blood dendritic cells (DCs) of chronic hepatitis B patients and its clinical significance. Methods Twenty patients with chronic hepatitis B (CHB) were randomly selected. Ten healthy controls were used as controls. Mononuclear cells were isolated from peripheral blood and cultured in vitro. Flow cytometry (FCM) was used to determine the phenotype of imDC and mDC. Flow cytometry and Western blotting were used to determine the expression of TLR3 on imDC and mDC, expression. Results In healthy controls, the expression rates of CD80, CD86 and HLA - DR and CD83 on the surface of mDC were (82.35 ± 8.67)%, (79.61 ± 10.08)% and (92.79 ± 8.48) % And (83.76 ± 5.47)%, respectively. The expression rates of imDC surface molecules were (28.31 ± 8.79)%, (31.17 ± 11.23)%, (27.61 ± 10.28)% and (23.46 ± 11.53)%, respectively (42.46 ± 9.22)%, (40.72 ± 11.24)%, (48.57 ± 12.51)%, (22.25 ± 11.22)% respectively in CHB group ) And (18.57 ± 10.22)%, (24.16 ± 10.46)%, (17.87 ± 10.38)% and (14.28 ± 6.77)%, respectively. There was no significant difference between the two groups (P> 0.05) The expression rate of the upper surface molecules of group mDC was significantly different from that of CHB group, but there was no significant difference in the expression rate of imDC upper surface between the two groups. The expression of TLR3 in imDC was significantly higher than that in mDC (P <0.01), while there was no significant difference in the expression of TLR3 between imDC and mDC in CHB patients (P> 0.05). The expression of TLR3 in imDC was significantly higher in CHB patients Lower than healthy controls (P <0.01). Conclusions The down-regulation of TLR3 expression in peripheral blood of CHB patients may be an important reason for the chronicity of HBV infection.