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用胚胎１６ｄ（ｅｍｂｒｙｏｎｉｃｄａｙ１６，Ｅ１６）、Ｅ１８、Ｅ２０、新生和成年Ｗｉｓｔａｒ大鼠各５只，取大脑、小脑、中脑、脑桥、延髓和脊髓组织制成细胞悬液，细胞悬液中，绝大部分细胞变为圆形，少数有突起的细胞经２—４ｈ的悬液培养突起回缩，细胞变圆后可顺利通过流式细胞计（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）上直径７０μｍ的喷嘴，证实ＦＣＭ可用于神经科学的研究，用ＦＣＭ测得Ｅ１６大鼠１万个细胞中Ｇ２／Ｍ期细胞数大脑明显高于小脑，Ｅ１８时大、小脑Ｇ２／Ｍ期细胞数达高峰，此时二者间无显著差异；Ｅ１６、Ｅ１８、Ｅ２０，中脑、脑桥、延髓和脊髓１万个细胞中Ｇ２／Ｍ期细胞数明显低于大、小脑。新生大鼠脑各部Ｇ２／Ｍ期细胞数急剧降低，成年与新生比无显著差异。
Six embryonic day 16 (E16), E18, E20 and five newborn and adult Wistar rats were used to prepare cell suspension in the brain, cerebellum, midbrain, pons, medulla oblongata and spinal cord. Some of the cells became round and a few of the cells with protrusions retracted after 2-4h suspension culture. After the cells became round, they passed smoothly through a nozzle with a diameter of 70μm on a flow cytometry (FCM), confirming that FCM could be used to Neuroscience research, using FCM measured E16 rat 10,000 cell G2 / M phase of the number of brain was significantly higher than the cerebellum, E18 large, cerebrum G2 / M phase cells reached the peak, at this time was no significant difference The number of cells in G2 / M phase of E16, E18, E20, midbrain, pons, medulla oblongata and spinal cord was significantly lower than that of large and cerebellum. Neonatal rat brain G2 / M phase of each cell number decreased sharply, no significant difference between adult and newborn.