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目的 :研究无细胞棒状杆菌纳米制剂 (Non cellNanoScaleProductfromCorynebacteriumParvum ,NCPP)对小鼠腹腔巨噬细胞 (PMφ)产生一氧化氮 (NO)的影响 ,探讨其抗肿瘤作用机制 ,为临床应用提供依据。方法 :采用小鼠腹腔注射NCPP与短棒状杆菌制剂 (CorynebacteriumParvumProduct ,CPP) ,1 0d后检测NO水平发现NCPP具有与CPP相同的作用。结果 :NCPP组和CPP组与对照组比较 ,检测结果显示 ,NCPP和CPP均可明显提高小鼠腹腔Mφ产生NO的水平 ,两组与对照组相比 ,有极显著性差异 (P <0 0 1 ) ,但NCPP和CPP两组之间无显著性差异 (P >0 0 5 )。结论 :NCPP与CPP具有相同作用 ,可激活巨噬细胞 ,分泌产生高水平NO以达到杀伤肿瘤细胞的目的
Objective: To study the effect of non cell NanoScaleProduct derived fromCorynebacteriumParvum (NCPP) on the production of nitric oxide (NO) in mouse peritoneal macrophages (PMφ) and to explore the mechanism of its anti-tumor effect, providing a basis for clinical application. Methods: NCPP and CorynebacteriumParvumProduct (CPP) were intraperitoneally injected into mice. NO levels were detected after 10 days and NCPP was found to have the same effect as CPP. Results: Compared with control group, NCPP group and CPP group showed that both NCPP and CPP significantly increased NO production in Mφ of mice peritoneal cavity, there was a significant difference between the two groups (P <0 0 1), but there was no significant difference between NCPP and CPP (P> 0.05). Conclusion: NCPP and CPP have the same effect, which can activate macrophages and produce high levels of NO to kill the tumor cells