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目的 探讨 +GZ暴露条件下大鼠脑中热休克蛋白 - 70 ( HSP70 )的变化及其在 +GZ所致脑损伤中的作用。 方法 10 0只大鼠 ,随机分为 4组 ,每组 2 5只 ,一组作为空白对照 ,其余三组分别进行 +2 GZ,+6 Gz和 +10 GZ暴露 ,峰值作用时间 3min,加速度增长率 1G/s。然后 ,分别于暴露后的 6 h、1d、2 d、4d和 6 d麻醉大鼠 ,心脏灌注后迅速取脑组织 ,用 West blot法比较 HSP70的变化情况。 结果 +GZ暴露后 6 h HSP70开始升高 ,1d达到高峰 ,至 6 d时基本恢复正常。不同 +GZ暴露之间进行比较可以看出 ,+6 GZ组 HSP70表达最高 ,+10 GZ最少。 结论 +GZ暴露对大鼠脑HSP70表达水平有显著的影响。
Objective To investigate the changes of heat shock protein 70 (HSP70) and its role in + GZ induced brain injury induced by + GZ in rats. Methods One hundred rats were randomly divided into 4 groups (25 rats in each group). One group served as blank control. The other three groups were exposed to +2 GZ, +6 Gz and +10 GZ respectively. The peak time was 3 min and the acceleration increased Rate 1G / s. Then, the rats were anesthetized 6 h, 1 d, 2 d, 4 d and 6 d after exposure, respectively. The brain tissue was taken immediately after cardiac perfusion. The changes of HSP70 were compared by West blot. Results The HSP70 began to increase 6 h after + GZ exposure, peaked at 1 d, and returned to normal after 6 d. Comparisons between different + GZ exposures show that HSP70 is the highest in + 6 GZ with the least in 10 GZ. Conclusion + GZ exposure has a significant effect on the expression of HSP70 in rat brain.